Again, another nugget of information I hunted around for and just couldn’t find. To alternate row styles, or more specifically, background colour…

dgv.RowsDefaultCellStyle.BackColor = Color.White;

Technically that should already be set from when you added the grid view, but I do it just to make sure. So now that the default is set, we can specify the alternate colour…

dgv.AlternatingRowsDefaultCellStyle.BackColor = Color.Gray;

If you don’t want to use the standard set colours, you can always specify your own…

dgv.AlternatingRowsDefaultCellStyle.BackColor = Color.FromArgb(240, 240, 240);